Ultraconserved element data for phylogenomic placement of the jumping spider genus Iranattus Prószyński, 1992 (Salticidae, Plexippini, Plexippina)

The jumping spider genus Iranattus Prószyński, 1992 has been placed in the Harmochirina based on morphology. We use phylogenomic evidence based on ultraconserved element  (UCE) data to show that it belongs instead in the subtribe Plexippina., We used target enrichment UCE sequencing, dual-indexed TruSeq-style libraries were prepared following methods used previously (e.g. Maddison et al. 2020b). Targeted enrichment using the RTA_v2 probeset (Zhang et al. 2023) was performed using the myBaits v. 4.01 protocol (Arbor Biosciences, <https://arborbiosci.com/wp-content/uploads/2023/06/myBaits_Manual_v5.03.pdf>). Libraries were sequenced on partial lanes of illumina NovaSeq 6000 S4 runs with 150-bp paired end reads.
Raw demultiplexed reads were processed with PHYLUCE v. 1.6 (Faircloth 2016), quality control and adapter removal were performed with Illumiprocessor wrapper (Faircloth 2013), and assemblies were created with SPAdes v. 3.14.1 (Nurk et al. 2013) using options at default settings. The UCE loci were recovered using RTA_v2 probeset (Zhang et al. 2023). The recovered loci were aligned with MAFFT using L-INS-i option (Katoh and Standley 2013). The aligned UCE loci were then trimmed with Gblocks (Castresana 2000, Talavera..., , # Phylogenomic placement of the jumping spider genus *Iranattus* Prószyński, 1992 (Salticidae, Plexippini, Plexippina)

[https://doi.org/10.5061/dryad.ht76hdrpz](https://doi.org/10.5061/dryad.ht76hdrpz)

The dataset includes (1) separate ultraconserved element (UCE) matrices and (2) concatenated matrices used in the phylogenomic analysis.

## Description of the data and file structure

(1) A NEXUS file (Iranattus-MaratheEtAl-SeparateMatrices.nex.zip) containing separate UCE matrices of 3398 loci recovered from SPAdes assemblies, Gblocks trimmed, re-aligned, and RAxML inferred gene trees for these UCE loci for paralogy test.

(2) A NEXUS file (Iranattus-MaratheEtAl-Concatenated.nex.zip) concatenated dataset of 3104 UCE matrices after deletion of paralogous loci based on branch lengths and loci represented in fewer than 10 taxa. This file also contains 10 maximum-likelihood trees and 1000 bootstrap trees inferred using IQ-TREE.