Huntingtin-RNA complex generation for cryoEM analysis 2019/07/24

<strong>Project: </strong>High resolution structural analysis of HTT-nucleic acid complexes <strong>Experiment: </strong>Huntingtin-RNA complex generation for cryoEM analysis <strong>Date: </strong>2019/07/24 <strong>Background: </strong>Huntingtin has been shown to copurify with nucleic acid material as well as bind different nucleic acid species in band shift assays. To more thoroughly characterise this interaction, HTT-nucleic acid complex generation and structure solution should provide insight into how huntingtin performs this function. <strong>Rationale: </strong>Previous attempts to capture HTT-nucleic complexes have been difficult and maintaining the complex throughout purification of the sample has been tricky. These four approaches are different ways to try and form a stable complex between Q54 HTT-HAP40 and CAG₈RNA.