Regulation by LTTRs LrhA, YafC, YdcI, and YhaJ in Escherichia coli K12

LrhA, YafC, YdcI, and YhaJ are LysR-type transcription regulator in Escherichia coli K12. In this study the transcriptomes of E. coli K12 strain U4 (MG1655 derivative, rph+ flhDC(IS1) dgcJ∷IS1 crl::IS1 ilvG+ ΔlacZ; Yilmaz et al. 2020, J. Bacteriol 203 e00427-20, https://doi.org/10.1128/JB.00427-20) and isogenic ΔlrhA, ΔyafC, ΔydcI, and ΔyhaJ mutants grown in Tryptone medium to OD600 0.8 were determined. The data revealed few loci that may be specifically affected in one deletion mutant only, while most differentially expressed loci were up or down regulated in several of the mutants. RNA-sequencing analyses were performed of five different E. coli K12 strains in three biological replicates each. The strains included E. coli K12 MG1655 derivative U4 (Yilmaz et al. 2020, J. Bacteriol 203 e00427-20, https://doi.org/10.1128/JB.00427-20) as reference strain and isogenic ΔlrhA, ΔyafC, ΔydcI, and ΔyhaJ deletion mutants.