Digital RNA with pertUrbation of Genes (DRUG-Seq) of LN-229 glioblastoma cells treated with 20 different drugs at two time points

DRUG-Seq is a miniatruized and multiplexed RNA-Seq protocol described in Ye et al., 2018 (https://www.nature.com/articles/s41467-018-06500-x) To determine transcriptional changes upon drug perturbation in LN-229 glioblastoma cells, we profiled 20 drug responses across two time-points (6 and 22 hours) by DRUG-Seq Overall design: These 20 drugs were selected to include pharmacoscopy-hit neuroactive drugs spanning diverse drug classes (n=11), pharmacoscopy-hit oncology drugs (n=7), pharmacoscopy-negative neuroactive drugs (n=2), and a DMSO control.