Aberrant Cell Fate and Functional Impairment in Developing Human Liver Organoids Exposed to Nanoplastics

Nanoplastics represent an emerging class of global environmental contaminants; however, their potential to interfere with human developmental processes remains largely unexplored. This study investigated the impact of exposure to polystyrene nanoplastics (PS-NP) on the de novo formation, differentiation, and function of human liver organoids derived from induced pluripotent stem cells (hiPSCs), a sophisticated model of early organogenesis. Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of liver organoids subjected to stepwise liver organoids differentiation with and without PS-NPs