Investigation of the Role of Micro-RNAs in the Immunopatho-genesis of Oral Lichen Planus Patients

Background and Objectives: Lichen planus (LP) is a chronic inflammatory disorder affecting the skin, mucous membranes, and other tissues. Its exact etiology remains elusive, but the immunopathogenesis of LP has gained significant attention due to its complex interplay between immune dysregulation and tissue response. We aim to investigate the importance of understanding LP immunopathogenesis, explicitly focusing on the role of microRNAs (miRNAs) in modulating the disease process. Materials and Methods: In our research, punch biopsy samples were collected from 85 patients, including 52 Oral Lichen Planus (OLP) and 33 healthy volunteer patients, according to specific inclusion and exclusion criteria. Samples were analyzed for miR-21, p53, and p63 expression using real-time Polymerase Chain Reaction (RT/PCR). Results: The OLP group was compared to a control group, revealing increased miR-21 and p53 expression.
Further comparison of the OLP group’s miR-21, p53, and p63 expression showed a weak positive correlation between p53 and miR-21 and a weak negative correlation between p63 and miR-21. These findings suggest that miR-21 and its potential targets, p53 and p63, play crucial roles in the pathogenesis and malignant potential of OLP. Conclusions: In conclusion, our research suggests that miR-21 and its target genes p53 and p63 are crucial in the molecular pathogenesis and cancerous potential of OLP. This understanding can lead to targeted treatment approaches for this pre-cancerous condition.

背景与目标：扁平苔藓（LP）是一种影响皮肤、粘膜及其他组织的慢性炎症性疾病。其确切病因尚无定论，然而，由于扁平苔藓的免疫病理机制涉及免疫失调与组织反应之间的复杂相互作用，其免疫病理机制的研究已受到广泛关注。本研究旨在探讨了解扁平苔藓免疫病理机制的重要性，特别是聚焦于微小RNA（miRNA）在调节疾病进程中的作用。材料与方法：本研究中，根据特定的纳入和排除标准，收集了85名患者的活检样本，包括52例口腔扁平苔藓（OLP）患者和33名健康志愿者。样本采用实时聚合酶链反应（RT/PCR）技术检测miR-21、p53和p63的表达。结果：将OLP组与对照组进行比较，结果显示miR-21和p53的表达增加。进一步比较OLP组的miR-21、p53和p63表达，发现p53与miR-21之间存在弱正相关，而p63与miR-21之间存在弱负相关。这些发现表明，miR-21及其潜在靶点p53和p63在OLP的发病机制和恶性潜能中发挥着至关重要的作用。结论：综上所述，本研究表明，miR-21及其靶基因p53和p63在OLP的分子发病机制和癌变潜力中扮演着关键角色。这一认识有望引导针对这一癌前状态的靶向治疗策略。