Effect of Lhx2 in K562 cells

Lhx2 is a LIM-homeobox transcription factor which could induce hematopoietic stem cell-like cells from mouse embryonic stem cells and induced pluripotent stem cells. However, the effects of Lhx2 overexpression in the human chronic myeloid leukemia cell line K562 remains unknown. Therefore we carried out Lhx2 overexpression in K562 cells. The overexpression of Lhx2 was performed using a retroviral vector carrying IRES-EGFP. Empty vector (EGFP) and the Lhx2 expression vector (Lhx2) were transduced into K562 cells and cultured for 2 days in RPMI/10%FCS. Then, EGFP+ transduced cells were isolated and cultured for an additional 3 days in RPMI/10%FCS.