Gene expression profiles of in vitro-infected (Leishmania major) and uninfected mouse bone marrow-derived macrophages. Mus musculus

Gene expression profiles of in vitro-infected (Leishmania major) and uninfected bone-marrow-derived macrophages from BALB/c, C57BL/6, C.B6-(lmr1, lmr2) and B6.c-(lmr1, lmr2) mouse strains. We utilised microarrays to investigate a number of issues. Firstly, we determined which genes were differentially regulated in macrophages in response to infection with Leishmania major. Secondly, we hoped to gain some insight into the differences between C-B6-(lmr1, lmr2) congenic mice and their parental control BALB/c, as well as differences between B6-c-(lmr1, lmr2) and their parental control C57BL/6. This would aid us in the search for the genes underlying our loci, since the only genomic regions differing between the two strains are those of the two congenic intervals on chromosomes 9 and 17. Any genes differentially regulated would be contributing to the experimental infection differences observed between the congenic mice and their parental controls. Overall design: L. major-infected and uninfected bone marrow-derived macrophages from C57BL/6, BALB/c, B6.c-(lmr1, lmr2) and C.B6-(lmr1, lmr2) mice were analyzed. Macrophages from 6 mice were pooled for each sample.

本数据集涵盖来自BALB/c、C57BL/6、C.B6-(lmr1, lmr2)及B6.c-(lmr1, lmr2)小鼠品系的、体外感染利什曼原虫（Leishmania major）与未感染的骨髓来源巨噬细胞（bone-marrow-derived macrophages）的基因表达谱。本研究采用基因芯片（microarrays）技术开展多项探究：其一，明确巨噬细胞在感染利什曼原虫后存在差异表达调控的基因；其二，解析C.B6-(lmr1, lmr2)同类系小鼠（congenic mice）与其亲本对照品系BALB/c之间的表达差异，同时探究B6.c-(lmr1, lmr2)同类系小鼠与其亲本对照品系C57BL/6之间的表达差异。由于两类品系间仅第9号和第17号染色体上的两个同类系导入区间存在基因组差异，该研究可助力我们定位相关基因位点背后的功能基因；而差异表达调控的基因将与同类系小鼠与其亲本对照间观察到的实验感染差异密切相关。实验整体设计：对来自C57BL/6、BALB/c、B6.c-(lmr1, lmr2)及C.B6-(lmr1, lmr2)小鼠的利什曼原虫感染与未感染骨髓来源巨噬细胞进行分析；每个样本均混合6只小鼠来源的巨噬细胞。