Characterisation and relative abundance of bacteria present in the urine of a frog

Reproductive technologies are increasingly being adopted to improve the Q6 conservation management of threatened species. The storage and transport of sperm is a vital aspect of the practical implementation of reproductive technologies, however, bacterial contamination during the storage and transport of sperm samples presents a biosecurity risk and can contribute to a reduction in sperm longevity during storage. The present study: 1) characterised the urinary microbiome (bacterial species composition and abundance) using culture-independent 16S rRNA sequencing; 2) quantified the effect of various doses of gentamicin and streptomycin-penicillin on bacterial abundance (colony-forming units; CFUs) and; 3) quantified the effect of antibiotic supplementation on the sperm viability (proportion live/dead) of spermic urine during a 12-day cold-storage period, using the common eastern froglet, Crinia signifera. Overall, urine samples were found to host a diverse array of bacteria, dominated by ..., , , # Urinary bacteria ID and relative abundance

[https://doi.org/10.5061/dryad.vt4b8gv0b](https://doi.org/10.5061/dryad.vt4b8gv0b)

Bacterial species composition and relative abundance present in urine samples collected from individual male Common eastern froglets, *Crinia signifera* (n=14). All frogs were collected from a single, large population, located adjacent to the Ecological Research Centre (ERC) at the University of Wollongong (34.4048° S, 150.8717° E). Collections occurred on October 19 and 26, 2022. Each row represents a species of bacteria identified to the finest scale that was able to be identified according to the SILVA rRNA database by the Australian Genome Research Facility. Unassigned species are species that were not able to be identified to taxonomic order.  Total absolute bacterial abundance is the sum of all absolute abundance raw reads counts for each species within each urine sample; data for each species is the relative abundance of each species within the sample,...