Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [miRNA Fibrosis Stages]. Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [miRNA Fibrosis Stages]

Human peripheral mononuclear cells were purified from control and diseased (Psedoexfoliation (PXF), Ocular Hypertension (OHT), Pseudoexfoliation Glaucoma (PXG)) patient volunteers blood using ficol and miRNA was extracted using QIAGEN miRNeasy kit Overall design: QPCR array profiling. PBMC from control and diseased patients (OHT and PXG) were collected as mentioned in summary followed by extraction of miRNA from the same prior to QPCR based miRNA expression array