Central Amygdala TRAP-Seq of Drd2 neurons

Sequencing actively translating transcripts of Drd2 neurons using translating ribosome affinity purification (TRAP identifies differentially regulated mRNAs following fear learning. Differentially expressed transcripts with potentially targetable gene products include Npy5r, Rxrg, Adora2a, Sst5r, Fgf3, ErbB4, Fkbp14, Dlk1 and Ssh3. TRAP-Seq of CeA Drd2 neurons following fear conditioning采用翻译核糖体亲和纯化技术（translating ribosome affinity purification, TRAP）对Drd2神经元的活跃翻译转录本进行测序，以鉴定恐惧学习后差异调控的mRNA。携带具备潜在靶向价值基因产物的差异表达转录本涵盖Npy5r、Rxrg、Adora2a、Sst5r、Fgf3、ErbB4、Fkbp14、Dlk1与Ssh3。本研究针对恐惧条件反射后的杏仁核中央区（Central Amygdala, CeA）Drd2神经元开展了TRAP-Seq测序实验。