PP2A complex disruptor SET prompts widespread hyper-transcription of growth-essential genes in the pancreatic cancer cells

Hyper-activation of the oncogenic transcription reflects the epigenetic plasticity of the cancer cells. SET was described as a nuclear factor that stimulated transcription from the chromatin template. However, the mechanisms of SET-dependent transcription are unknown. Here, we found that over-expression of SET and CDK9 induced very similar transcriptome signatures in multiple cancer cell lines. SET localized in the transcription start site (TSS)-proximal regions and supported the RNA transcription. SET specifically bound the PP2A-C subunit and induced PP2A-A subunit repulsion from the C subunit, which indicated the role of SET as a PP2A-A/C complex disruptor in the TSS-proximal regions. Through blocking PP2A activity, SET assisted CDK9 to maintain Pol II CTD phosphorylation and activated mRNA transcription. Our findings position SET as a key factor that modulates chromatin PP2A activity, promoting the oncogenic transcription in pancreatic cancer., , , # PP2A complex disruptor SET prompts widespread hyper-transcription of growth-essential genes in the pancreatic cancer cells

## Usage notes

This dataset contains the results of the SET protein complex analysis. It also provides the way to access other supplementary data of this research.

### Datasets included:

1\) SET protein complex analysis in the HEK293T cells

1. 10 million HEK293T cells were stably transfected with the control and Myc-SET plasmids, respectively.
2. Magnetic beads conjugated with anti-SET antibody were added to the control and SET-OE cell lyastes.
3. The control SET and SET-OE associated proteins were immunoprecipitated with magnetic beads and subjected to SDS-PAGE separation.
4. Thermo UltiMate 3000 UHPLC was utilized to separate samples of dried peptides. The liquid phase chromatography-separated peptides were ionized by a nanoESI source and passed to a tandem mass spectrometer Q-Exactive HF X for Data Dependent Acquisition mode detection.
5. For each d...