PIP3 functional analysis and subcellular localization of tumor-associated PTEN mutations.

* PIP3 in vivo activity in yeast was monitored by the reconstitution of the p110α-CAAX-induced lack-of-growth phenotype (+, reconstitution; +/-, partial reconstitution;-, no reconstitution). Data of mutations are on a PTEN 1–403 background, from Fig 2 and from [36].† Major subcellular localization (N, nucleus; C, cytoplasm; N/C, nucleus/cytoplasm) was determined by immunofluorescence on mammalian COS-7 cells. Data of mutations are on a PTEN 1–375 background, from Fig 2.# From COSMIC (http://cancer.sanger.ac.uk/cosmic) and HGMD (http://www.hgmd.cf.ac.uk) databases. Abbreviations: BD, bladder cancer; BRR, Bannayan-Riley-Ruvalcaba syndrome; CC, colon carcinoma; CS, Cowden syndrome; EC, endometrial cancer; Glb, glioblastoma; JPS, juvenile polyposis syndrome; LC, lung carcinoma; LDD, Lhermitte-Duclos disease; NHML, non-Hodgkin’s malignant lymphoma; OC, ovarian carcinoma; SC, stomach carcinoma; SS, synovial sarcoma. In italic, germ-line mutations.PIP3 functional analysis and subcellular localization of tumor-associated PTEN mutations.