Effect of culture system on gene expression during traditional 2D culture and 3D culture of mouse mesothelioma cells. Effect of culture system on gene expression during traditional 2D culture and 3D culture of mouse mesothelioma cells

To investigate the differences between mesothelioma- organoid culture and 2D traditional culture, we established 7 mesothelioma lines and 3 normal mesothelial cells from model mouse. We then performed gene expression profiling analysis using data obtained from RNA-seq of 20 different cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for mesothelioma cells and its culture system between 2D and 3D.