Establishing the copper response of Burkholderia cenocepacia K56-2

Purpose - To establish the response of B. cenocepacia K56-2 to growth in copper, and to characterise the BCAM0442/3 copper-sensing TCS. Methods - RNA-seq of the response WT and Δbcam0442/3 B. cenocepacia K56-2 to mid-log phase growth in LB with or without 1 mM CuCl2 was performed. Results - Characterisation of the overall response of B. cenocepacia K56-2 to copper, as well as establishing that BCAM0442/3 regulates the CopABCDE system in B. cenocepacia WT and Δbcam0442/3 B. cenocepacia K56-2 were grown for 8 hours in LB with or without 1 mM CuCl2. Total RNA was then extracted and processed. Three replicates per conditions.