Comprehensive transcriptome profiling for response to phytohormone-induced signaling in Capsicum annuum L.

We sought to investigate the scope of transcriptome analyses of peppers subjected to four major phytohormones. For this, At the 6-true-leaf stage, pepper plants were sprayed at underside of leaves with 5 mM sodium salicylate (SA), 100 μM methyl jasmonate (JA), 5 mM ethephone (ET), 100 μM (±)-ABA, or distilled water (mock). For RNA-seq library construction, the third or fourth leaves from four plants were harvested per replicate at 0, 1, 3, 6, 12, and 24 h after treatment. A total of 187.8 Gb of transcriptome data were generated using transcriptome analysis pipelines consisting of quality control, quantification, and differential gene expression analyses. The extensive transcriptome data obtained will provide valuable information for future studies of crops exposed to responsive of phytohormones. We performed the RNA-seq analyses of peppers treated with SA, JA, ET, and ABA at six different time points. A total of 78 RNA samples, containing three biological replicates per time point for each of the phytohormones and a mock control, were used for RNA-sequencing