Kinetic parameters of WT, Monomeric and Heteromultimeric CaMKII for ATP.
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The ATP kinetic parameters of WT-CaMKII, Δ317-α-CaMKII and heteromeric (α-I205K)-(β-K43R) CaMKII are given. The quantity of CaMKII used for the assays was: WT-CaMKII-0.01–0.017 µg; Monomeric Δ317-α-CaMKII- (0.15 µg); Heteromeric (α-I205K)-(β-K43R) CaMKII-1.9 µg. For heteromeric CaMKII assay, saturating concentrations of purified preparations of GST-GluN2A (6.5 µM) and GST-GluN2B (9.4 µM) were used. For the rest of the assays saturating concentrations of fusion proteins in bacterial lysates were used. p values were calculated in comparison with corresponding GluN2A data in each set of experiments. pFig. S1.
创建时间：
2015-12-02
