Molecular amplification systems used in this study.

Oligonucleotides sequences (primers and probes) are presented with their corresponding species, gene targets and amplicon sizes. Illumina Nextera universal tails sequences that have been added to primers during the PCR amplification step of the metabarcoding method are represented in green and barcodes in blue. An adenine (A) nucleotide was added between the barcode and the primer (not mandatory). (XLSX)