Expression profiling in breast cancer brain metastases compared to breast cancers and primary brain tumors

Experiment: Expression profiling in breast cancer brain metastases (BC) compared to breast cancers (BC) and primary brain tumors (prBT). The objectives are to identify expression profiles that are specific to BCBM in order to identify new molecular biomarkers. The characterization of the BCBM samples included adjacent genetic techniques. We extracted RNA from BCBM and prBT. RNA samples were processed and hybridized to Affymetrix microarrays. Microarray expression samples from BC were used from one of our previous study. Bioinformatics tools and adjacent techniques were employed to display BCBM specific expression profiles and other genetic characteristics.