File S1 - Strengths and Limitations of 16S rRNA Gene Amplicon Sequencing in Revealing Temporal Microbial Community Dynamics

The file includes Figures S1 to S6 and Table S1 to S4. Figure S1 Dissolved oxygen and temperature profiles for July–December 2009 at the Brown’s Bridge location of Lake Lanier. Figure S2 Number of OTUs identified in each dataset. Figure S3 Similarity of datasets based on shared OTUs. Figure S4 Differences in phylum abundance based on 16S and metagenomes. Figure S5 Differences in abundance of individual genera based on 16S and metagenomes. Figure S6 Abundance of an individual Burkholderia-like population based on 16S amplicon vs. 16S metagenomic reads. Table S1 Characteristics of Lake Lanier at Brown’s Bridge for several dates in 2009. Table S2 V1, V3 specific primers used for amplification of the 16S rRNA gene. Table S3 16S rRNA gene amplicon library statistics. Table S4 metagenome library statistics. (PDF)