supplementary figures and Tables from Cultured cells and wing disc size of silkworm can be controlled by the Hippo pathway

Hippo signalling represents a cell proliferation and organ-size control pathway. Yorki (Yki), a component of the Hippo pathway, induces the transcription of a number of targets that promote cell proliferation and survival. The functions of <i>Yki</i> have been characterized in Drosophila and mammal, while there are few reports on silkworm, <i>Bombyx mori</i>. In the present study, we found that <i>BmYki3</i> facilitates cell migration and cell division, and enlarges the cultured cell and wing disc size. CoIP results indicated that BmYki3 may interact with thymosin, E3 ubiquitin-protein ligase, protein kinase ASK1, dedicator of cytokinesis protein 1, calcium-independent phospholipase A2 and beta-spectrin. RNA-seq results indicated that 4444 genes were upregulated and 10291 genes were downregulated after <i>BmYki3</i> was overexpressed in the cultured cells. GO annotation indicated that the up/downregulated genes were enriched in 268/382 GO terms (<i>p</i> &lt; 0.01); KEGG analysis showed that the up/downregulated genes were enriched in 49/101 pathways. These findings provided novel information to understand the functions of <i>BmYki3</i> in a cell proliferation and organ-size control pathway.