mouse lung endothelial single cell transcriptomics

Disruption of pulmonary vascular homeostasis is a central feature of viral pneumonia, wherein endothelial cell (EC) death and subsequent angiogenic responses represent critical determinants of the outcome of severe lung injury. A more granular understanding of the fundamental mechanisms driving reconstitution of the lung endothelium is necessary to facilitate therapeutic targeting of vascular repair. Here, we applied single-cell RNA sequencing (scRNA-seq) to profile lung ECs from mice on D0, D20 and D30 post influenza infection. Our data revealed the dynamics of endothelial subsets during influenza injury. Overall design: Influenza virus A/H1N1/PR/8 was administered intranasally at 50 to 60 TCID50 units to C57BL/6 mice as our previously described (Zhao et al, 2020). Mouse lung ECs sorted from uninjured (D0) and on 20 and 30 days after influenza infection.