ROR1-targeted delivery of miR-29b results in epigenetic reprogramming with p21-dependent cell cycle arrest

RNAseq analysis of purified splenic CD19+ B lymphocytes from the hRORxTCL1 mouse model treated with 2A2-miR-29b-ILP or 2A2-scramble-ILP. Moreover, 128 of the 233 differentially expressed genes are related to cell growth and proliferation. Overall design: Gene expression profiling of engrafted murine leukemia identified reprogramming of cell cycle regulators with decreased SP1 and increased p21 expression following miR-29b treatment. The experiment was further confirmed by protein modulation leading to cell cycle arrest.