Genomic assembly of Botyrococcus braunii race B (Metzger et al. 1988) Ayame strain

This dataset is a genomic assembly (v1.0) of DNA from a culture of
Botryococcus braunii race B, Ayame strain. The strain was isolated in 1984
by Metzger et al Phytochemistry (1988), 27, 1383-1388. The site of
isolation was Cote d'Ivoire, barrier lake of Ayame, 24th Feb 1984,
pH5.7 water temp 30.8.  The strain was then cultured in a lab for
25 years until being selected for this genomic denovo assembly
project. The strain makes biofuels consisting of methylated triterpenoids
termed "botryococenes." The project objectives and achievements
were to selectively identify and assemble full length triterpenoid
synthase genes, annotate them, determine the numer of paralogues
in this set of genes, and to investigate their evolution,
via a bioinformatic process. The annotations of the slected genes
are housed elsewhere on NCBI as genbank accessions KU248134,
KU248135, KU248136 and KU248137, while the rest of the assembly
is documented here. Genes in the assembly are from across the
entire genome, and commensals that were present in the cultutre
are also deliberately represented in the datset aswell. The lab leading
the publication (Ball lab) is a bioremediation lab, and the commensals are
useful remediation organisms. Further, genes upstream in the triterpenoid
synthesis pathway othere than the terminal genes that were
targeted, are in the datset and are of interest to the
wider molecular microbiology community.