<i>P<sub>r</sub></i> promoter activity at different induction levels of Rco<sub>LS20</sub>.
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Overnight culture of GR92 cells grown in the absence of IPTG was diluted 100-fold in fresh prewarmed LB medium containing the indicated amount of IPTG at 37°C. βGA was determined for samples withdrawn at late exponential growth (0D600 = ∼0.8). Background levels obtained with negative control strain PKS7 were <0.1 MU. Values are the mean of three independent experiments and for each point fluctuations were less than 10%. βGA, β galactosidase activity. Pr promoter activity at different induction levels of RcoLS20.
创建时间：
2014-10-23
