Indexing of selected symptomatic barley samples by next generation sequencing.
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NGS analyses were performed on highly purified double-stranded RNAs extracted from 21 barley leaf samples. The viruses identified are indicated, with the percentages of total reads mapping to their genome; PCR and NGS results are compared for BaYMV and BaMMV screening. BaYMV type, either Y1 (BaYMV-1) or Y2 (BaYMV-2), was determined from codon at position 132 of VPg in NGS data and in parallel with the dCAPS assay.
创建时间:
2017-11-29



