Differences in whole kidneys and renal F4/80+ macrophages in mice with the major and minor allele of the rs3184504 polymorphism after hypertension induction

To determine differences in kidney and renal F4/80+ macrophage gene expression between mice homozygous for the Arg-encoding versus the Trp-encoding rs3184504 polymorphism after 4 weeks of angiotensin II infusion. Overall design: Crispr-Cas9 was used to create mice homozygous for the Arg-encoding major allele and the Trp-encoding minor allele of the rs3184504 polymorphism. Kidneys and renal F4/80+ macrophages were isolated from these mice after 4 weeks of angiotensin II infusion to induce hypertension.