IC₅₀ values of antibabesial drugs evaluated for bovine <i>Babesia</i> and equine <i>Babesia</i> and <i>Theileria</i> parasites using fluorescence- and microscopy-based methods without daily replacement of the medium with 2.5% and 5% HCTs, respectively.

a IC50 values for each drug were calculated on the fourth day based on the growth inhibitions determined using fluorescence- and microscopy-based methods in three separate experiments. Each drug concentration was made in triplicate in each experiment, and the final obtained IC50s were the mean of values obtained from three separate experiments. b The differences between the IC50 values calculated using fluorescence- and microscopy-based methods were not statistically significant for all six drugs analyzed. *P < 0.05 significant differences between the drug-treated and diminazene aceturate groups. **P ≤ 0.01 very significant differences between the drug-treated and diminazene aceturate groups. ***P ≤ 0.0001 extremely significant differences between the drug-treated and diminazene aceturate groups. IC50 values of antibabesial drugs evaluated for bovine Babesia and equine Babesia and Theileria parasites using fluorescence- and microscopy-based methods without daily replacement of the medium with 2.5% and 5% HCTs, respectively.