Data for: Experimental Analysis of Lifelines in a 15,000 L Bioreactor by Means of Lagrangian Sensor Particles

The DaRUS repository entails the raw files, result files and the MATLAB codes for the publication "Experimental Analysis of Lifelines in a 15,000 L Bioreactor by Means of Lagrangian Sensor Particles" in the journal "Chemical Engineering Research and Design". This study employs Lagrangian Sensor Particles (LSPs) with a diameter of 40 mm equipped with a pressure sensor to investigate cell lifelines in a 15,000 L stirred tank reactor (STR) with three Elephant Ear impellers. The Stokes number of the LSPs is approx. 0.004 on a macro-scale. The vertical probability of presence, axial velocity profiles, circulation time distributions, and residence time distributions are quantified to analyze single-phase mixing heterogeneities, detect hydrodynamic compartments and conduct a Lagrangian regime analysis. Results reveal a similarly distributed probability of presence in the vertical reactor center but emphasize the LSP's sensitivity to fluctuating densities. Axial velocity distributions illustrate characteristic impeller-induced flow patterns, and circulation time distributions identify three compartments with comparatively shorter times in the axial center. Residence time distributions exhibit a similar compartmentalized profile. Moreover, the study estimates a potential oxygen deprivation zone for CHO cells in the upper compartment and demonstrates the LSP's efficacy in characterizing impeller systems. Contrary to literature, the ratio of examined global mixing times to circulation times is 1.0, highlighting macro-scale mixing. The research underscores that LSPs offer crucial insights into industrial-scale STRs, specifically for determining hydrodynamic compartments without having optical access.

DaRUS数据仓库包含了《通过拉格朗日传感器颗粒对15,000升生物反应器中生命线进行实验分析》一文的原始文件、结果文件以及MATLAB代码，该文发表于《化学工程研究与应用设计》期刊。本研究采用直径为40毫米并配备压力传感器的拉格朗日传感器颗粒（LSPs）来探究15,000升搅拌式反应器（STR）中细胞的生命线，该反应器配备三个象耳式叶轮。在宏观尺度上，LSPs的斯托克斯数约为0.004。通过量化垂直存在概率、轴向速度分布、循环时间分布和停留时间分布，本研究分析了单相混合的异质性，检测了流体动力学隔室，并进行了拉格朗日状态分析。结果显示，在垂直反应器中心存在概率分布均匀，但强调了LSP对密度波动的敏感性。轴向速度分布展示了叶轮诱导的典型流动模式，而循环时间分布识别出轴向中心具有相对较短时间的三个隔室。停留时间分布呈现出类似的隔室化特征。此外，研究估计了CHO细胞在上部隔室中的潜在缺氧区域，并证明了LSP在表征叶轮系统中的有效性。与文献中的描述相反，所考察的全局混合时间与循环时间的比率达到1.0，突出了宏观尺度的混合。研究强调，LSPs为工业规模STRs提供了关键的洞察，特别是在没有光学访问的情况下确定流体动力学隔室方面。