Single cell transcriptomc analysis of CD34+ cells from primary myelofibrosis patient

We performed single cell RNA sequencing by means of 10X Genomics platform of CD34+ hematopoietic stem/progenitor cells (HSPCs) from primary myelofibrosis (PMF). Cells were isolated through immunomagnetic selection from frozen PBMCs coming from one patient with PMF who evolved to Acute Myeloid Leukemia (AML). Three timepoints were considered: chronic phase (Time1), after 8 months of Ruxolitinib treatment (Time2) and after 11 months of Ruxolitinib treatment at AML diagnosis (Time3). The main purpose of this project was to reconstruct the composition of the neoplastic clone in terms of HSPCs subpopulations at different time-points during disease evolution and to study changes in gene expression within each cell cluster over time. This dataset include 3 samples from the same patient affected by PMF. The first sample correspond to the chronic phase of the disease. The second one is taken 8 month after Ruxolitinib treatment initiation. The third sample was collected 11 month after Ruxolitinib treatment initiation at the time of AML diagnosis.