Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: Sanger Impact of PCR on Chimera Formation
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下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP295727
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资源简介：
The impact of PCR conditions on chimera content was evaluated by dideoxy sequencing the HMP even Mock community, targeting the V3-V5 region. The following PCR parameters were tested in addition to the original protocol: lower cycle number (20x), and longer extension time (10 min.)
创建时间：
2020-12-03
