Epigenome analysis of muscle tissue and paired cultured myoblasts from the Hertfordshire Sarcopenia Study extension

Genome wide DNA methylation profiling of paired muscle tissue and cultured myoblast genomic DNA. The Illumina Infinium Methylation450 array was used to obtain DNA methylation profiles across approximately 450,000 CpGs. Samples were male, taken from the Hertfordshire Sarcoepnia Study exension (HSSe), to determine prelimnary associations measures of muscle mass, function and insulin/glucose levels and DNA methylation in muscle tissue. Furthermore, we sought to determine whether DNA methylation in the muscle tissue is conserved in cultured myoblasts from the muscle biopsies and whether association differed between the two tissues. Bisulphite converted DNA from the 37 samples were hybridised to the Illumina Human Infinium Methylation450 array