Col-HA defect images

Data associated with article: Effects of cell-attachment and extracellular matrix on bone formation in vivo in collagen-hydroxyapatite scaffolds Max M. Villa, Liping Wang, David W. Rowe, and Mei Wei Department of Materials Science & Engineering, 97 North Eagleville Road, University of Connecticut, Storrs, CT 06269 Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 This dataset contains histological sections of critical size calvarial defects filled with collagen-hydroxyapatite scaffolds and culture expanded bone marrow osteoprogenitors after three weeks in vivo. Naming scheme: Group_AnimalEarNotch-Slide#_replicate#Channel#+Channel#+Channel#.jpg example: G1-NB-1_s1c2.jpg Explanation: Group G1 - samples incubated in vitro overnight before implantation G2 - samples incubated in vitro overnight before implantation +BME gel used as a seeding suspension G3 -samples implanted immediately after seeding G4 -samples implanted immediately after seeding +BME gel used as a seeding suspension AnimalEarNotch G1&G2 NB - ear notch both NL - ear notch left NR - ear notch right HR - hole right HL - hole left HB - hole both G3&G4 NB - ear notch both NL - ear notch left NR - ear notch right HR - hole right HL - hole left HB - hole both Slide Number Three tissue sections were placed on a slide. Slide number can be a value 1, 2 or 3. Replicate number One the replicate serial sections of the same calvarium, can be a value s1, s2 or s3. Channel number Corresponds to 1 of the 3 channels acquired: Channel1-c1- DIC, grayscale Channel2-c2- calcein mineralization label, green Channel3-c3- Tdtomato, donor cells, red