Male autism spectrum disorder is linked to brain aromatase disruption by prenatal BPA in multimodal investigations and 10HDA ameliorates the related mouse phenotype (10HDA)

Primary cortical neurons were obtained from male Cyp19-EGFP mouse embryos at gestational day 15.5. Embryos were genotyped for SRY to determine sex, and only male embryos were used. Cells were seeded in 24-well plates containing 12 mm glass coverslips, coated with 100 µg/mL poly D-lysine to a density of ~0.45 x 106 cells/well and incubated in a humidified CO2 incubator (5% CO2, 37°C). Primary cortical from Cyp19-EGFP mouse embryos were obtained on GD 15.5. Neuronal cell cultures were treated with vehicle or 1mM 10HDA (Matreya, PA ,USA) To investigate the effect of 10HDA on RNA expression in cortical primarly cell culture (obtained from Cyp19-EGFP mice at gestational day 15.5), we treated primary cortical neurons obtained from mice with either vehicle (DSMO) or 1mM 10HDA.