Figure S1 - Competitive sugar inhibition assay from Monitoring extracellular vesicle surface glyco-properties using fluorescent lectins and nanoparticle tracking analysis

Panel A - Results of the inhibition of WGA and RCA I binding to tested seminal EVs (sEVs) isolates by N-acetylglucosamine (GlcNAc) or galactose (Gal), demonstrating complete inhibition. Panel B - Share of lectin-positive sEVs before and after inhibition with the corresponding monosaccharide. Concentrations of lectin-labeled sEVs without sugar were defined as 100%, and post-inhibition values were expressed as a percentage of that baseline. Numbers above each column indicate the corresponding mean percentage values ± standard deviation, from three independent measurements. UC-sEVs, seminal EVs isolated by ultracentrifugation; UC-SEC-sEVs, seminal EVs isolated by ultracentrifugation and size exclusion chromatography; UC-MF-sEVs, seminal EVs isolated by ultracentrifugation and microfiltration; WGA, wheat germ agglutinin; RCA I - Ricinus communis agglutinin.