Drug-metabolizing activity of CYP2C93 protein determined using human CYP2C substrates.

CPR, cytochrome P450 reductase. N.D., not determined.In each reaction, 5 pmol of the recombinant protein was used with substrate (50 µM diclofenac, 100 µM flurbiprofen, 100 µM paclitaxel, 200 µM S-mephenytoin, or 1 mM tolbutamide) as described in Materials and Methods.The recombinant cynomolgus and rhesus monkey CYP2C93 proteins were analyzed along with cynomolgus monkey CYP2C8, CYP2C43, CYP2C75, and CYP2C76. CYP2C93v1 and CYP2C93v2 correspond to SV1 and SV2 transcripts of CYP2C93, respectively.