MicV, VrrA sRNA target identification

In this study we determined the target spectra of the MicV and VrrA sRNAs via pulse-expression followed by RNA-sequencing. Overall design: mRNA profiles of V.cholerae ?micV ?vrrA strains carrying pBAD-ctr (empty vector control), pBAD-micV or pBAD-vrrA (inducible sRNA expression vectors). The bacteria were grown to early stationary phase, expression of the sRNAs was induced by addition of L-arabinose, and RNA-samples were taken 10minutes after induction. Three indepent biological replicates for each condition are provided.