Integrated transcriptomics and proteomics analyses reveal anthelmintic mechanism of arctigenin against Gyrodactylus kobayashii

The widespread resistance of parasitic worms to commonly used anthelmintics highlights the urgent need for development of new effective chemicals. Arctigenin (ARC) is a natural lignin compound that exhibits excellent anthelminthic efficacy against infections of Gyrodactylus, but knowledge about the mechanism of action is far from clear. In the present study, high-throughput RNA-sequencing was integrated with iTRAQ quantitative proteomics analysis to explore the anthelmintic mechanism and possible molecular targets of ARC against Gyrodactylus kobayashii.