Validation of a physiological relevant control medium.
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Cleavage rate at day 2 p.i., number of formed blastocysts at day 7 p.i. relative to the number of matured oocytes or to the number of cleaved zygotes. Oocytes (n = 595; three replicates) were matured in standard serum-free maturation medium devoid of all fatty acids [59] and in physiologically relevant, NEFA containing maturation medium with physiological, non toxic NEFA concentrations ( = control medium used in this study: 150 µM of total NEFA, i.e. OA, SA and PA). Data marked with different superscripts per row are significantly different between treatments (P
创建时间:
2015-12-02



