A sexually dimorphic hepatic cycle of very low density lipoprotein uptake and assembly

Single-cell transcriptomes can reveal spatiotemporal programmes of liver function on the sublobular scale. However, how sexual dimorphism affects this space-time logic remains poorly understood. To address this, we performed single-cell RNA-seq in the mouse liver, which allowed us to model how lobular position, circadian time and sex shape the transcriptome. Overall design: Mice were perfused with collagenase via the inferior vena cava, livers isolated, and cells released into medium and enriched for hepatocytes with a centrifugation step. The cells were then frozen. Upon thawing, sequencing libraries for scRNA-seq were constructed using 10x Genomics Chromium v3.