Proteome analysis of NPH3-domain and asapartic protease mutants exposed to long term moderate drought stress

Untargeted proteomic analysis was conducted to analyze the effect of longer term (4 days) low water potential (drought) stress treatment on proteome content of Arabidospsis thaliana Col-0 wild type and four sets of mutants or transgenic lines. A mutant (nrl5-2) with reduced expression of the NPH3-domain protein NRL5 has an extreme stress sensitive phenotype. A quadruple mutant of four aspartic proteases (aprd4) also has stress sensitive phenotype and possible effect on drought signaling. Transgenic plants having overexpression of one of these proteases were also analyzed. Note that another transgnenic line that was analyzed (NRL5_OX) was later found to have been mis-genotyped and this data is not being used for further analysis in our laboratory.