Escherichia coli strain:Chi7122 Genome sequencing and assembly. Escherichia coli strain:Chi7122

Previous studies have used Protein Glycan Coupling Technology to generate a live-attenuated avian pathogenic Escherichia coli (APEC) strain capable of N-glycosylation of target proteins using a chromosomally integrated Campylobacter jejuni pgl locus. However this proved ineffective against challenge. In this study we demonstrate the lack of surface exposure of glycosylated protein in APEC strain chi7122 carrying the pgl locus. Furthermore, we hypothesize that this may be due to the complex cell-surface architecture of E. coli. To this end, we removed the lipopolysaccharide O-antigen of APEC chi7122 pgl+ via deletion of the wecA gene and demonstrate increased surface exposure of glycosylated antigens (NetB and FlpA) in this strain.