GLs .xlsx

Glucosinolate content was analyzed using 4 weeks
old plants. Five plants of each
line were used as biological replicates. Plants were cut from top of the soil,
put in liquid nitrogen to stop chemical reactions and transferred to
freeze-drier for 3 days. Freeze-dried samples were finely ground using
micro-dismemberator U (B. Braun Biotech International). Finely grounded
material was sent to NIOO for dedicated glucosinolate analysis. Briefly,
samples were boiled in 70% MeOH followed by washing two times in 1 ml of 70% MeOH and
two times with 1 mL MQ water
and one times in 2mL of NaOAC 16 hr
ahead of treatment with 20 μl arylsulfatase. Following arylsulfatase
treatment freeze dried elute was redissolved in 1mL MQ water and filtered over
a 20 μm filter. Separation and detection of glucosinolates were performed using
reversed phase C-18 column and photodiode array detector respectively.
Glucosinolates measurement and concentration calculation was done following the
procedure of Kabouw et al., (2010). A list of measured glucosinolates and their
abbreviated names are given in