Identifying Novel Molecules Implicated in Early Mesendoderm Specification by a Broad Transcriptomic Approach

H1 embryonic Stem Cells maintained in pluripotency by Activin A and induced with BMP4 H1 cells were adapted to Activin A based mTeSR for at least 10 passages. The cells were subsequently split in 3 full 6-well plates and incubated in the same medium until they reached approximately 50% confluency. At that point, the cells were induced with 50ng/mL BMP4 for 1hr or 4hrs. A full 6-well plate served as a non-induced control. RNA was extracted from all the conditions with the experiment being repeated 3 times using cells from three consecutive passages for statistical significance.