Additional file 1: Table S1. of Use of a bioinformatic-assisted primer design strategy to establish a new nested PCR-based method for Cryptosporidium

Relevant information pertaining to small subunit ribosomal RNA gene (SSU) sequences obtained from amplicons produced from selected faecal DNA samples using a nested PCR method ([2]; see Methods section). These DNA samples were employed to assess the analytical specificity of large subunit ribosomal RNA gene (LSU) nested PCR assay established in this study (see also Methods section) (cf. Fig. 1). Sequence similarities (90–100%) were calculated with reference to the closest matched sequence in the GenBank database using BLASTn. Table S2. Pairwise comparison of sequence difference (%) in the variable D8 domain of the large subunit of the nuclear ribosomal RNA gene (LSU) used for the construction of the phylogenetic tree. Table S3. Pairwise comparison of sequence difference (%) in the region of the small subunit of the nuclear ribosomal RNA gene (SSU) used for the construction of the phylogenetic tree. Table S4. Salient information pertaining to the sequences of the variable D8 domain of the nuclear large subunit ribosomal RNA gene (LSU) used for the construction of the phylogenetic tree (cf. Fig. 2a). Sequences produced in this study are shown in bold-type. Table S5. Salient information pertaining to the sequences from the small subunit of the nuclear ribosomal RNA gene (SSU) used for the construction of the phylogenetic tree (cf. Fig. 2b). Sequences produced in this study are shown in bold-type. (XLSX 78 kb)