Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut

We used an experimental model of Salmonella infection to investigate the role of Lactobacillus paracasei CNCM I-5220-derived postbiotic to protect from leaky gut. Mice were treated orally with 135mg/kg/day of LP-PBF during 10 days (n=8 per group). Control mice received the vehicle control containing maltodextrins and FOS. After pretreatment, mice were infected with Salmonella typhimurium strain SL3261AT via oral gavage and after 6h were sacrificed and fecal pellet was collected at three time points (T0 Beginning of the experiment, T1 upon pre-treatment with LP-PBF or vehicle, TF upon Salmonella infection and sacrifice). DNA extracted from fecal pellets was analyzed using 16S rRNA gene sequencing.