Rat renal cell atlas and perturbation by losartan characterized with single-cell and spatial transcriptome information. Rattus norvegicus strain:Sprague Dawley, from Charles River

To identify the local changes of the gene expression patterns by administration of losartan at single cell level, we performed both single cell RNA sequencing (scRNA-seq) and spatial transcriptome sequencing (ST-seq) of the kidney from the rat treated with or without losartan. The sequencing library for scRNA-seq were prepared by Chromium Single Cell 3 Library Kit v2 (10x Genomics) and that for ST-seq were prepared by Visium Spatial Gene Expression Slide & Reagents Kit (10x Genomics). Following the integration analysis of scRNA-seq and ST-seq, we detected the spatially restricted changes of the gene expression patterns by administration of losartan in several cell types, such as macula densa cells or juxtaglomerular cells. We further analyze the ST-seq data in combination with the deconvolution tool, using scRNA-seq as a reference, and consequently observed spatial heterogeneity in the response to losartan among the glomeruli.