RNA-seq of THP-1 cells stimulated with CpG-c and Hydroxychloroquine intervention

Purpose: To investigate the inhibition of inflammatoty cytokines of Hydroxychloroquine to phagocytic THP-1 cells. Methods: we established a cell model of hemophagocytosis by simulating THP-1 cells with CpG-c for 24h in vitro to produce macrophages, and then coculture the cells with human RBCs. RNA sequencing analysis of THP-1 cells were performed to find Gene changed with CpG-c stimulation and Hydroxychloroquine intervention. Results:CpG-c sitimulation significantly altered gene expression of THP-1 cells. Cytokine genes were upregulated, especially a significant increase in IL-6 level from scratch. HCQ significantly inhibited the expression of inflammatory cytokines and decreased IL-6 gene level to baseline. Conclusions:Hydroxychloroquine can inhibite cytokine genes, especially IL-6 Gene level of THP-1 cells caused by CpG-c sitimulation. THP-1 cells (5 × 10e5 cells) were cultured with 3 μM CpG-c (ODN-2395) or PBS for 24 h to produce macrophages(CpG-c group) or control THP-1 cells(control group). Human RBCs (1 × 10^7 cells) were added to the system to perform haemophagocytosis for 3 h. Hydroxychloroquine (10 mg/μL) was administered to inhibit phagocytosis for 3 h(CpG-HCQ group).