Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes

To identify molecular characteristics of mouse AMs stimulated with or without Poly(I:C) in the presence of vehicle or MSDC overnight in vitro, we isolated RNA from AMs with various treatments and examined by bulk RNA-seq. We found a large number of cytokine gene profiles were reduced following MSDC treatment in WT AMs, suggesting that MSDC treatment recuced inflammatory response in AMs. Examined with bulk RNA-seq of mouse AMs stimulated with or without Poly(I:C) in the presence of vehicle or MSDC overnight in vitro for overnight ------------------------- The authors state that they have lost the raw data.