CenhANCER

Here, we developed CenhANCER, a comprehensive cancer enhancer database, based on H3K27ac ChIP-Seq datasets of 805 primary cancer tissues and 671 cancer cell lines across 49 cancer types. In total, we identified 29,815,953 typical enhancers and 516,447 super enhancers (SE) for the primary tissues, and 27,213,455 typical enhancers, 461,964 super enhancers for the cell lines. To strengthen the regulatory roles of the super-enhancers, we further integrated expression quantitative trait locus (eQTLs) , and risk SNPs into CenhANCER. We also identified transcription factors, whose binding sites were enriched for enhancers in each sample, which are potential therapeutic targets.本研究基于覆盖49种癌症类型的805份原发性癌组织与671株癌细胞系的组蛋白H3K27乙酰化染色质免疫共沉淀测序（H3K27ac ChIP-Seq）数据集，构建了综合性癌症增强子数据库CenhANCER。总计，我们为原发性癌组织鉴定出29,815,953个典型增强子与516,447个超级增强子（super enhancers, SE），为癌细胞系鉴定出27,213,455个典型增强子与461,964个超级增强子。为强化超级增强子的调控功能注释，我们进一步将表达数量性状基因座（expression quantitative trait locus, eQTLs）与风险单核苷酸多态性位点（risk SNPs）整合至CenhANCER数据库中。此外，我们还鉴定出在各样本的增强子区域中结合位点显著富集的转录因子，此类转录因子均为潜在治疗靶点。

搜集汇总

背景与挑战

背景概述

CenhANCER是一个综合性癌症增强子数据库，基于805个原发癌组织和671个癌细胞系的H3K27ac ChIP-Seq数据，覆盖49种癌症类型。它识别了数千万个典型增强子和数十万个超级增强子，并整合了eQTLs、风险SNPs以及转录因子信息，以强化调控作用并探索潜在治疗靶点。